2017-12-15 17:11:262025-05-22 17:51:30
description
Transcriptional activator of the gltA-gltB operon. Activates expression of the operon in the absence of arginine.
transcriptional activator/ repressor of the [[gene|gltA]]-[[gene|gltB]] operon, activates expression of the operon in the absence of arginine, represses in the presence of glutamate
locus
BSU18460
BSU_18460
geneLength
900
903
function
positive regulation of the glutamate synthase operon (gltAB)
regulation of the glutamate synthase operon ([[gene|gltA]]-[[gene|gltB]])
product
transcriptional regulator (LysR family)
transcriptional regulator ([SW|LysR family])
outlinks
bsu
BSU18460
BSU_18460
Gene
Coordinates
2,014,779 → 2,015,681
2,014,779 2,015,681
The protein
Catalyzed reaction/ biological activity
transcription activation of the ''[[gene|gltA]]-[[gene|gltB]]'' operon [Pubmed|2548995]
transcription activation of the [[gene|gltA]]-[[gene|gltB]] operon [Pubmed|2548995]
The protein
Protein family
[SW|LysR family] [Pubmed|2548995]
[SW|LysR family] (according to UniProt) [Pubmed|2548995]
The protein
Paralogous protein(s)
none, but there are 19 members of the LysR family in ''B. subtilis
[[this]]
The protein
[SW|Domains]
DNA-binding helix-turn-helix motif: AA 18 ... 37
DNA-binding helix-turn-helix motif: AA 18 ... 37
[SW|HTH lysR-type domain] (aa 1-58) (according to UniProt)
Biological materials
Mutant
GP344 (erm), (available in [SW|Jörg Stülke]'s lab)
GP738 (gltC::Tn10, spc), (available in [SW|Jörg Stülke]'s lab)
GP1904 (''ΔgltC''::''aphA3''), (available in [SW|Jörg Stülke]'s lab) [pubmed|28294562]
BKE18460 (Δ[[gene|gltC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGC
BKK18460 (Δ[[gene|gltC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGC
GP344 (erm), (available in [SW|Jörg Stülke]'s lab)
GP738 (gltC::Tn10, spc), (available in [SW|Jörg Stülke]'s lab)
GP1904 (''gltC''::''aphA3''), (available in [SW|Jörg Stülke]'s lab) [pubmed|28294562]
BKE18460 ([[gene|gltC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGC
BKK18460 ([[gene|gltC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGC
Biological materials
Expression vector
for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP903, available in [SW|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with C-terminal Strep-tag, in pET3C: pGP951, available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[SW|Fabian Commichau] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]
References
Original Publications
The protein
Structure
[PDB|2H99] (from Acinetobacter baylyi, corresponds to aa 1 - 245, 35% identity) [pubmed|19400783]
The protein
additional information
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000048 PRODORIC2 database]
Biological materials
Expression vectors
for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP903, available in [SW|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with C-terminal Strep-tag, in pET3C: pGP951, available in [SW|Jörg Stülke]'s lab
labs
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[SW|Fabian Commichau] Senftenberg, Germany [http://www.uni-goettingen.de/de/413008.html homepage]